De novo synthesis and desaturation of fatty acids at the mitochondrial acyl‐carrier protein, a subunit of NADH:ubiquinone oxidoreductase in Neurospora crassa

FEBS Letters

Husmann, Heribert¹  Weiss, Hanns²  Schneider, Regina²  Zensen, Ralf²  Peine, Thorsten² 

[1] Max-Planck-Institut für Kohleforschung, Mühlheim a.d Ruhr, Germany;Institut für Biochemie, Universität Düsseldorf, Universitätsstaße 1 4000 Düsseldorf 2, Germany

关键词: NADH:ubiquinone oxidoreductase;    Complex I;    Mitochondrial fatty acid synthesis;    Cardiolipin;   

DOI  :  10.1016/0014-5793(92)81324-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have cultivated the cel mutant of Neurospora crassa defective in cytosolic fatty acid synthesis with [2-¹⁴C]malonate and found radioactivity covalently attached to the mitochondrial acyl-carrier protein (ACP), a subunit of the respiratory chain NADH:ubiquinone oxidoreductase. We purified the ACP by reverse-phase HPLC: the bound acyl groups were trans-esterified to methylesters and analyzed by gas chromatography. The saturated C₀ to C₁₈ fatty acids and oleic acid were detected. De novo synthesis and desaturation of fatty acids at the ACP subunit of NADH:ubiquinone oxidoreductase and use of the products of this mitnchondrial synthetic pathway for cardiolipin synthesis is discussed.

【 授权许可】

Unknown