FEBS Letters | |
Incorporation of 1‐deoxy‐d‐xylulose into isoprene and phytol by higher plants and algae | |
Müller, Christian1  Gröner, Rainer1  Lichtenthaler, Frieder W2  Zeidler, Johannes1  Focke, Manfred1  Schwender, Jörg1  Braun, Siegmar2  Lichtenthaler, Hartmut K1  | |
[1] Botanisches Institut, Universität Karlsruhe, D-76128 Karlsruhe, Germany;Institut für Organische Chemie, Technische Universität Darmstadt, D-64287 Darmstadt, Germany | |
关键词: Deoxy-d-xylulose; Isopentenyl diphosphate; Isoprene; Isoprenoid biosynthesis; Mevalonate; Phytol; Sterol; dXu; 1-deoxy-d-xylulose; GAP; d-glyceraldehyde-3-phosphate; GC-MS; gas chromatography-mass spectrometry; IPP; isopentenyl diphosphate; Me-[1-2H1]-dXu; methyl-[1-2H1]-1-deoxy-α/β-d-xyluloside; MVA; mevalonic acid; NMR; nuclear magnetic resonance; TLC; thin layer chromatography; | |
DOI : 10.1016/S0014-5793(97)01002-8 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
In further substantiating the novel mevalonate-independent pathway for isoprenoid biosynthesis, which generates isopentenyl diphosphate (IPP) via 1-deoxy-d-xylulose-5-phosphate, labeling experiments with 1-[2H1]deoxy-d-xylulose were performed with various higher plants and algae: efficient incorporation was observed into isoprene emitted by Populus, Chelidonium, and Salix, into the phytol moiety of chlorophylls in a red alga (Cyanidium), in two green algae (Scenedesmus, Chlamydomonas), and a higher plant (Lemna). By contrast, 13C-mevalonate applied was incorporated into isoprene and phytol to a much lower extent or not at all. This demonstrates that this `1-deoxy-d-xylulose-5-phosphate pathway' for biosynthesis of plastidic isoprenoids is widely distributed in photosynthetic organisms.
【 授权许可】
Unknown
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