Incorporation of 1‐deoxy‐d‐xylulose into isoprene and phytol by higher plants and algae

FEBS Letters

Müller, Christian¹  Gröner, Rainer¹  Lichtenthaler, Frieder W²  Zeidler, Johannes¹  Focke, Manfred¹  Schwender, Jörg¹  Braun, Siegmar²  Lichtenthaler, Hartmut K¹ 

[1] Botanisches Institut, Universität Karlsruhe, D-76128 Karlsruhe, Germany;Institut für Organische Chemie, Technische Universität Darmstadt, D-64287 Darmstadt, Germany

关键词: Deoxy-d-xylulose;    Isopentenyl diphosphate;    Isoprene;    Isoprenoid biosynthesis;    Mevalonate;    Phytol;    Sterol;    dXu;    1-deoxy-d-xylulose;    GAP;    d-glyceraldehyde-3-phosphate;    GC-MS;    gas chromatography-mass spectrometry;    IPP;    isopentenyl diphosphate;    Me-[1-2H1]-dXu;    methyl-[1-2H1]-1-deoxy-α/β-d-xyluloside;    MVA;    mevalonic acid;    NMR;    nuclear magnetic resonance;    TLC;    thin layer chromatography;   

DOI  :  10.1016/S0014-5793(97)01002-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In further substantiating the novel mevalonate-independent pathway for isoprenoid biosynthesis, which generates isopentenyl diphosphate (IPP) via 1-deoxy-d-xylulose-5-phosphate, labeling experiments with 1-[²H₁]deoxy-d-xylulose were performed with various higher plants and algae: efficient incorporation was observed into isoprene emitted by Populus, Chelidonium, and Salix, into the phytol moiety of chlorophylls in a red alga (Cyanidium), in two green algae (Scenedesmus, Chlamydomonas), and a higher plant (Lemna). By contrast, ¹³C-mevalonate applied was incorporated into isoprene and phytol to a much lower extent or not at all. This demonstrates that this `1-deoxy-d-xylulose-5-phosphate pathway' for biosynthesis of plastidic isoprenoids is widely distributed in photosynthetic organisms.

【 授权许可】

Unknown