The iridoid glucoside secologanin is derived from the novel triose phosphate/pyruvate pathway in a Catharanthus roseus cell culture

FEBS Letters

Lefeber, Alfons W.M¹  Contin, Adriana²  Verpoorte, Robert¹  van der Heijden, Robert² 

[1] Division of NMR, Leiden Institute of Chemistry, Gorlaeus Laboratories, P.O. Box 9502, 2300 RA Leiden, The Netherlands;Division of Pharmacognosy, Leiden/Amsterdam Center for Drug Research (LACDR), Gorlaeus Laboratories, P.O. Box 9502, 2300 RA Leiden, The Netherlands

关键词: Secologanin;    Iridoid;    Alkaloid;    Biosynthesis;    Isopentenyl diphosphate;    1-Deoxy-d-xylulose;    MVA-5PP;    mevalonate-5 diphosphate;    IPP;    isopentenyl diphosphate;    DMAPP;    dimethylallyl diphosphate;    GPP;    geranyl diphosphate;   

DOI  :  10.1016/S0014-5793(98)01022-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Secologanin is the iridoid building block of the majority of the terpenoid indole alkaloids. In the biosynthesis of secologanin, mevalonate was considered to be the exclusive precursor of isopentenyl diphosphate. After [1-¹³C]glucose feeding to a cell culture of Catharanthus roseus, its incorporation into secologanin was studied by ¹³C NMR spectroscopy. The data showed that the novel triose phosphate/pyruvate and not the mevalonate pathway was the major route for the biosynthesis of secologanin.

【 授权许可】

Unknown