Cell‐cycle‐dependent phosphorylation of the basal transcription factor RAP74

FEBS Letters

Yonaha, Masatomo¹  Tsuchiya, Terumasa¹  Yasukochi, Yukio¹ 

[1]Department of Molecular Genetics, Medical Research Institute, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113, Japan

关键词: TFIIF;    RAP74;    Cell cycle;    Phosphorylation;    TF;    transcription factor;    TAF;    TBP-associated factor;   

DOI  :  10.1016/S0014-5793(97)00642-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In this report, cell-cycle-dependent effects of TFIID on other basal transcription factors were investigated. We purified TFIID fractions from HeLa cells synchronized in the S/G2 phases and in early G1 phase, and show that RAP74 is phosphorylated more highly by the S/G2 phase TFIID fraction than by the early G1 phase TFIID fraction. Further analyses using deletion mutants of RAP74 revealed that amino acid residues 206–256 are phosphorylated by the TFIID fraction. Reconstitution of in vitro transcription activity indicates that the cell-cycle-dependent phosphorylation of RAP74 increases TFIIF transcription activity.

【 授权许可】

Unknown