Oscillation of ADP‐ribosyl cyclase activity during the cell cycle and function of cyclic ADP‐ribose in a unicellular organism, Euglena gracilis

FEBS Letters

Inageda, Kiyoshi³  Nishina, Hiroshi³  Tsuyama, Shingo¹  Katada, Toshiaki⁴  Takahashi, Katsunobu⁵  Miyatake, Kazutaka²  Masuda, Wataru²  Takenaka, Shigeo⁶  Inui, Hiroshi²  Nakano, Yoshihisa² 

[1] Department of Veterinary science, Osaka Prefecture University, Sakai, Osaka 593, Japan;Department of Applied Biological Chemistry, Osaka Prefecture University, Sakai, Osaka 593, Japan;Department of Life Science, Tokyo Institute of Technology, Yokohama 227, Japan;Department of Physiological Chemistry, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113, Japan;National Institute of Neuroscience, NCNP, Kodaira, Tokyo 187, Japan;Laboratory of Nutrition and Food Science, Hagoromo-gakuen College, Sakai, Osaka 592, Japan

关键词: ADP-ribosyl cyclase;    Cyclic ADP-ribose;    Cell cycle;    Ca2+ release;    Euglena gracilis;    cADPR;    cyclic adenosine diphosphoribose or cyclic ADP-ribose;    Cbl;    cobalamin;    PMSF;    phenylmethylsulfonyl fluoride;   

DOI  :  10.1016/S0014-5793(97)00168-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In Euglena gracilis, the activity of ADP-ribosyl cyclase, which produces cyclic ADP-ribose, oscillated during the cell cycle in a synchronous culture induced by a light-dark cycle, and a marked increase in the activity was observed in the G2 phase. Similarly, the ADP-ribosyl cyclase activity rose extremely immediately before cell division started, when synchronous cell division was induced by adding cobalamin (which is an essential growth factor and participates in DNA synthesis in this organism) to its deficient culture. Further, cADPR in these cells showed a maximum level immediately before cell division started. A dose-dependent Ca²⁺ release was observed when microsomes were incubated with cADPR. © 1997 Federation of European Biochemical Societies.

【 授权许可】

Unknown