Human lymphocyte antigen CD38 catalyzes the production of cyclic ADP‐ribose

FEBS Letters

Jackson, David G.²  Summerhill, Robin J.¹  Galione, Antony¹ 

[1]University Department of Pharmacology, Mansfield Road, Oxford, OX1 3QT, UK

[2]Department of Molecular Immunology, Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, OX3 9 DU, UK

关键词: Cyclic ADP-ribose;    CD38;    ADP-ribosyl cyclase;    NAD+ glycohydrolase;    NADase;   

DOI  :  10.1016/0014-5793(93)80735-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The human lymphocyte antigen CD38 has been shown to share sequence homology with ADP-ribosyl cyclase, the enzyme that catalyzes the conversion of NAD⁺ to cyclic ADP-ribose (cADPR), a potent Ca²⁺-mobilizing agent. In this study COS1 cells from African Green Monkey kidney were transiently transfected with CD38 cDNA, inducing expression of authentic CD38 on the cell surface. We demonstrate that CD38 expressed in this manner can convert NAD⁺ to cADPR in the extracellular medium as assessed by Ca²⁺ release from sea-urchin egg microsomes.

【 授权许可】

Unknown