Modified nucleotides as substrates and inhibitors of adenylate kinase from different sources

FEBS Letters

Chernov, Dmitry N²  Krayevsky, Alexander A²  Frank-Kamenetskaya, Maria D¹  Skoblov, Juri S² 

[1] Institute of Molecular Genetics, Russian Academy of Sciences, 1 Kurchatov Square, Moscow 123182, Russian Federation;Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov str., Moscow 117984, Russian Federation

关键词: Adenylate kinase;    Modified nucleotide;    Phosphorylation;    Substrate specificity;   

DOI  :  10.1016/0014-5793(96)01039-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The substrate and inhibitory properties of modified nucleotides with respect to adenylate kinase from rabbit muscles, human placenta and Escherichia coli were studied. A number of 5′-hydrogenphosphonates and 5′-fluorophosphates of modified nucleotides were shown to inhibit the phosphorylation reaction catalyzed by these enzymes. A clear difference between phosphonates of 3′-deoxyribonucleotides and the corresponding ribo- and 2′,3′-dideoxyribonucleotides was found. 3′-Azido-2′,3′-dideoxythymidine and its phosphorus derivatives did not inhibit the adenylate kinase reaction.

【 授权许可】

Unknown