Subcellular localization of annexin V in human foreskin fibroblasts: nuclear localization depends on growth state

FEBS Letters

Barwise, Jennifer L.¹  Walker, John H.¹ 

[1] Department of Biochemistry and Molecular Biology, University of Leeds, Leeds LS2 9JT, UK

关键词: Annexin V;    Growth state;    Nucleus;    Fibroblasts;   

DOI  :  10.1016/0014-5793(96)00950-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Annexin V is a major intracellular calcium-binding protein in human foreskin fibroblasts. Immunocytochemistry revealed that annexin V was localized in the nucleus and throughout the cytoplasm in human foreskin fibroblasts. The presence of annexin V in the nucleus was variable depending on the growth state. Nuclear staining was strongest in proliferating cells immediately after sub-culture, and decreased on prolonged culture without changing the culture medium. The cytoplasmic location of annexin V was not greatly affected by the same conditions. Refeeding cells with fresh serum restored annexin V to the nuclei of all cells within in 24 h indicating that nuclear localization of annexin V is dependent on serum factors.

【 授权许可】

Unknown