Interaction of heparin with annexin V

FEBS Letters

Mealy, Tanya R²  VanderNoot, Victoria A¹  Capila, Ishan³  Linhardt, Robert J³  Seaton, Barbara A² 

[1] Department of Chemical and Biochemical Engineering, University of Iowa, Iowa City, IA 52242, USA;Structural Biology Group, Department of Physiology, Boston University School of Medicine, Boston, MA 02118, USA;Division of Medicinal and Natural Products Chemistry, College of Pharmacy, University of Iowa, Iowa City, IA 52242, USA

关键词: Heparin;    Surface plasmon resonance;    Annexin V;    Octasaccharide;    Isothermal titration calorimetry;   

DOI  :  10.1016/S0014-5793(99)00245-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The energetics and kinetics of the interaction of heparin with the Ca⁺² and phospholipid binding protein annexin V, was examined and the minimum oligosaccharide sequence within heparin that binds annexin V was identified. Affinity chromatography studies confirmed the Ca⁺² dependence of this binding interaction. Analysis of the data obtained from surface plasmon resonance afforded a K _d of ∼21 nM for the interaction of annexin V with end-chain immobilized heparin and a K _d of ∼49 nM for the interaction with end-chain immobilized heparan sulfate. Isothermal titration calorimetry showed the minimum annexin V binding oligosaccharide sequence within heparin corresponds to an octasaccharide sequence. The K _d of a heparin octasaccharide binding to annexin V was ∼1 μM with a binding stoichiometry of 1:1.

【 授权许可】

Unknown