Demonstration of human Borna disease virus RNA in human peripheral blood mononuclear cells

FEBS Letters

Zhong, Qiu⁴  Nakamura, Yurie⁴  Kato, Shiro¹  Kakinuma, Mitsuaki⁴  Ikuta, Kazuyoshi⁴  Nakaya, Takaaki⁴  Senjo, Masanori²  Kishi, Masahiko⁴  Ikeda, Kazuhiko³ 

[1] Osaka University, Suita, Osaka 565, Japan;Health Sciences University of Hokkaido, Ishikari, Japan;Tokyo Institute of Psychiatry, Setagaya-ku, Tokyo 000, Japan;Institute of Immunological Science, Hokkaido University, Kita-ku, Sapporo 060, Japan

关键词: Borna disease virus;    RT-PCR;    Peripheral blood mononuclear cell;    BDV;    Borna disease virus;    RT-PCR;    reverse transcriptase-polymerase chain reaction;    PBMC;    peripheral blood mononuclear cells;    GST;    glutathione S-transferase;   

DOI  :  10.1016/0014-5793(95)00406-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

BDV naturally infects horses and sheep, and causes sporadic neurological disease. Serological evidence suggests an association of BDV, or a related virus, with specific psychiatric diseases in humans. Here, by using a nested RT-PCR technique, we demonstrate that human BDV RNA is present in the PBMC of psychiatric patients. In an examination of a total of 60 patients from 5 wards of a hospital in Japan, the detection rate differed within each ward, ranging from 8% to > 50% (37% on the average). Of particular note was the finding that the human derived BDV sequences, which included deleted forms in about 23% of the positive samples, were slightly different from those derived from horse BDV. These results suggest urgent consideration of the measures to be taken to cope with the effects of blood transfusion. In addition, the detection of a high level of BDV in the PBMC of patients will help our understanding of the pathogenesis in the disease.

【 授权许可】

Unknown