Demonstration of Borna disease virus RNA in peripheral blood mononuclear cells derived from Japanese patients with chronic fatigue syndrome

FEBS Letters

Takahashi, Hirokazu³  Nakamura, Yurie³  Asahi, Sayumi³  Kitani, Teruo¹  Ikuta, Kazuyoshi³  Nakaya, Takaaki³  Yamanishi, Koichi²  Tobiume, Minoru³  Kuratsune, Hirohiko¹ 

[1] Department of Hematology and Oncology, Osaka University School of Medicine, Suita, Osaka 565, Japan;Department of Microbiology, Osaka University School of Medicine, Suita, Osaka 565, Japan;Section of Serology, Institute of Immunological Science, Hokkaido University, Kita-ku, Sapporo 060, Japan

关键词: Borna disease virus;    Chronic fatigue syndrome;    Peripheral blood mononuclear cell;    RT-PCR;    CFS;    chronic fatigue syndrome;    BDV;    Borna disease virus;    RT-PCR;    reverse transcriptase-polymerase chain reaction;    PBMC;    peripheral blood mononuclear cell;    GST;    glutathione S-transferase;   

DOI  :  10.1016/0014-5793(95)01439-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

CFS, a recently named heterogeneous disorder, is an illness of unknown etiology. The association of CFS with viral infections has been suggested. A common association between CFS and several viruses examined has not been confirmed. Here, we centered on the possible link between CFS and BDV infection. By nested RT-PCR followed by hybridization, BDV RNA was demonstrated as a clear signal in PBMCs in 3 out of 25 CFS patients. The amplified cDNA fragments were cloned and sequenced. A total of 16 clones were studied. Intra-patients divergencies of the p24 were 2–9%, 3–20%, and 3–11% in the deduced amino acids. Inter-patient divergencies among the 16 clones were 3–24%. Antibodies to recombinant BDV p24 protein were detected in 6 CFS patients including one carrying BDV RNA. Overall, these gave the prevalence of 32% (8/25) in Japanese CFS patients, suggesting that Japanese CFS is highly associated with active infection of BDV, or a related agent.

【 授权许可】

Unknown