International Journal of Molecular Sciences | |
Real-Time qPCR Identifies Suitable Reference Genes for Borna Disease Virus-Infected Rat Cortical Neurons | |
Lujun Zhang2  Siwen Liu1  Liang Zhang1  Hongmin You1  Rongzhong Huang3  Lin Sun1  Peng He1  Shigang Chen1  Hong Zhang1  Peng Xie2  | |
[1] Chongqing Key Laboratory of Neurobiology, Chongqing 400016, China; E-Mails:;Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, China; E-Mail:;Department of Rehabilitation, the Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China; E-Mail: | |
关键词: Borna disease virus; BDV; reference gene; RT-qPCR; cortical neuron; | |
DOI : 10.3390/ijms151221825 | |
来源: mdpi | |
【 摘 要 】
Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) is the most commonly-used technique to identify gene expression profiles. The selection of stably expressed reference genes is a prerequisite to properly evaluating gene expression. Here, the suitability of commonly-used reference genes in normalizing RT-qPCR assays of mRNA expression in cultured rat cortical neurons infected with Borna disease virus (BDV) was assessed. The expressions of eight commonly-used reference genes were comparatively analyzed in BDV-infected rat cortical neurons and non-infected control neurons mainly across 9 and 12 days post-infection. These reference genes were validated by RT-qPCR and separately ranked by four statistical algorithms: geNorm, NormFinder, BestKeeper and the comparative delta-
【 授权许可】
CC BY
© 2014 by the authors; licensee MDPI, Basel, Switzerland.
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