Identification of the amino terminal subunit of the glycoprotein of Borna disease virus

FEBS Letters

Kraus, Ina¹  Kiermayer, Simone¹  Richt, Jürgen A²  Eickmann, Markus¹  Garten, Wolfgang¹ 

[1] Institut für Virologie der Philipps-Universität Marburg, Robert-Koch-Straße 17, D-35037 Marburg, Germany;Institut für Virologie am Fachbereich Veterinärmedizin der Justus-Liebig-Universität Giessen, Frankfurter Stra. 107, D-35392 Giessen, Germany

关键词: Borna disease virus;    Glycoprotein;    Amino terminal subunit;    Carbohydrate masking;    Blot deglycosylation;    BDV;    Borna disease virus;    ConA;    concanavalin A;    Endo H;    endoglycosidase H;    GP;    glycoprotein precursor (gp84/gp94);    GP-C;    carboxy terminal glycoprotein subunit (gp43);    GP-N;    amino terminal glycoprotein subunit (gp51);    GNA;    Galanthus nivalis agglutinin;    PNGase F;    peptide-N-glycosidase F;    Rb-αGP-C;    rabbit antiserum against carboxy terminal BDV GP;    Rb-αGP-N;    rabbit antiserum against amino terminal BDV GP;    Vero cells;    African green monkey kidney cells;    WGA;    wheat germ agglutinin;   

DOI  :  10.1016/S0014-5793(02)03513-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The only surface membrane glycoprotein of Borna disease virus (BDV) is synthesized as a polypeptide with a molecular mass of 57 kDa and N-glycosylated to a precursor glycoprotein (GP) of about 94 kDa. It is processed by the cellular protease furin into the C-terminal membrane-anchored subunit GP-C, also known as gp43, and a presumptive N-terminal subunit GP-N, that is highly glycosylated and has a molecular mass of about 51 kDa. However, up to now the latter remained undetected in BDV-infected material. We describe a novel approach to identify glycan masked linear antigenic epitopes. In the present study, GP-N was identified in BDV-infected cells by a combination of lectin precipitation, enzymatic deglycosylation on blot and immunochemistry using an N-terminal specific antiserum. The GP-N has an apparent molecular mass of 45–50 kDa in its glycosylated form and 27 kDa in its deglycosylated form. N-glycan analysis revealed that the precursor GP contains only mannose-rich N-glycans, whereas GP-N and GP-C contain mannose-rich and complex-type N-glycans.

【 授权许可】

Unknown