FEBS Letters | |
Identification of the amino terminal subunit of the glycoprotein of Borna disease virus | |
Kraus, Ina1  Kiermayer, Simone1  Richt, Jürgen A2  Eickmann, Markus1  Garten, Wolfgang1  | |
[1] Institut für Virologie der Philipps-Universität Marburg, Robert-Koch-Straße 17, D-35037 Marburg, Germany;Institut für Virologie am Fachbereich Veterinärmedizin der Justus-Liebig-Universität Giessen, Frankfurter Stra. 107, D-35392 Giessen, Germany | |
关键词: Borna disease virus; Glycoprotein; Amino terminal subunit; Carbohydrate masking; Blot deglycosylation; BDV; Borna disease virus; ConA; concanavalin A; Endo H; endoglycosidase H; GP; glycoprotein precursor (gp84/gp94); GP-C; carboxy terminal glycoprotein subunit (gp43); GP-N; amino terminal glycoprotein subunit (gp51); GNA; Galanthus nivalis agglutinin; PNGase F; peptide-N-glycosidase F; Rb-αGP-C; rabbit antiserum against carboxy terminal BDV GP; Rb-αGP-N; rabbit antiserum against amino terminal BDV GP; Vero cells; African green monkey kidney cells; WGA; wheat germ agglutinin; | |
DOI : 10.1016/S0014-5793(02)03513-5 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The only surface membrane glycoprotein of Borna disease virus (BDV) is synthesized as a polypeptide with a molecular mass of 57 kDa and N-glycosylated to a precursor glycoprotein (GP) of about 94 kDa. It is processed by the cellular protease furin into the C-terminal membrane-anchored subunit GP-C, also known as gp43, and a presumptive N-terminal subunit GP-N, that is highly glycosylated and has a molecular mass of about 51 kDa. However, up to now the latter remained undetected in BDV-infected material. We describe a novel approach to identify glycan masked linear antigenic epitopes. In the present study, GP-N was identified in BDV-infected cells by a combination of lectin precipitation, enzymatic deglycosylation on blot and immunochemistry using an N-terminal specific antiserum. The GP-N has an apparent molecular mass of 45–50 kDa in its glycosylated form and 27 kDa in its deglycosylated form. N-glycan analysis revealed that the precursor GP contains only mannose-rich N-glycans, whereas GP-N and GP-C contain mannose-rich and complex-type N-glycans.
【 授权许可】
Unknown
【 预 览 】
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RO201912020312395ZK.pdf | 160KB | download |