Inhibition of arginase byin alveolar macrophages: implications for the utilization of l‐arginine for nitric oxide synthesis

FEBS Letters

Racké, Kurt²  Hey, Claudia²  Hecker, Markus¹  Nematollahi, Heideh²  Busse, Rudi¹ 

[1] Center of Physiology, Johann Wolfgang Goethe University Clinic, Theodor-Stern-Kai 7, D-60590 Frankfurt am Main, Germany;Center of Pharmacology, Johann Wolfgang Goethe University, Frankfurt am Main, Germany

关键词: NO synthase;    Arginase;    Hydroxyarginine;    Arginine;    Macrophage;    Lipopolysaccharide;    E. coli;   

DOI  :  10.1016/0014-5793(95)00039-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The hypothesis was investigated that the nitric oxide (NO) synthase intermediate, (HOArg), is an arginase inhibitor in rabbit or rat alveolar macrophages. Exogenously applied HOArg strongly inhibited the arginase activity present in these cells (IC₅₀ ≥ 15 μM), and attenuated l-[³H]arginine transport (IC₅₀ ≥ 500 μM) in rabbit alveolar macrophages. Moreover, up to 37 μM HOArg were detected in the conditioned medium, but not in the lysate, of rat alveolar macrophages exposed to bacterial lipopolysaccharide for 18 h. HOArg may thus be a potent endogenous arginase inhibitor in these cells which increases the availability of l-arginine for NO biosynthesis.

【 授权许可】

Unknown