l‐Arginine depletion by arginase reduces nitric oxide production in endotoxic shock: an electron paramagnetic resonance study

FEBS Letters

Shergill, J.K.²  Cook, H.T.¹  Bune, A.J.¹  Cammack, R.² 

[1] Department of Histopathology, St. Marys Hospital Medical School, Imperial College of Science, Technology and Medicine, Norfolk Place, London W2 1PG, UK;Centre for the Study of Metals in Biology and Medicine, Division of Life Sciences, King's College, University of London, Campden Hill Road, London W8 7 AH, UK

关键词: Nitric oxide;    Arginase;    Lipopolysaccharide;    Endotoxic shock;    Arginine;    NOS;    nitric oxide synthase;    l-NMMA;    LPS;    lipopolysaccharide;    DETC;    diethyldithiocarbamate;    EPR;    electron paramagnetic resonance;   

DOI  :  10.1016/0014-5793(95)00495-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nitric oxide (NO) synthesis was measured in the liver, lung, spleen and kidney of lipopolysaccharide-treated male rats using the nitric oxide spin trap, iron (II)-diethyldithiocarbamate (FeDETC₂). Nitric oxide formation in vivo was determined by the increase in intensity of the characteristic triplet hyperfine EPR spectrum of [NO-FeDETC₂]. Intravenous bovine liver arginase, at a dose which completely depleted circulating arginine, significantly reduced the formation of nitric oxide in these tissues. The general decrease in NO levels was confirmed by the decrease in plasma nitrite levels. These results directly demonstrate that NO formation in endotoxic shock depends on extracellular arginine; depletion of plasma arginine may be a useful therapeutic strategy.

【 授权许可】

Unknown