The inhibitory effect of nitrite, a stable product of nitric oxide (NO) formation, on arginase

FEBS Letters

Temesi, Ágnes¹  Mészáros, G.¹  Bajor, T.¹  Hrabák, A.¹ 

[1] Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University Medical School, VIII. Puskin u. 9., POB 260, H-1444 Budapest, Hungary

关键词: Arginase;    NO synthase;    Nitrite;    Inhibition;    Fluorescence;    Macrophage;    FBS;    foetal bovine serum;    DMEM;    Dulbecco's minimal essential medium;    IFNγ;    interferon-γ;    IL;    interleukin;    NOS;    NO synthase;    TLC;    thin-layer chromatography;    TNFα;    tumor necrosis factor-α;    amino acids are abbreviated by their usual three-letter symbols;   

DOI  :  10.1016/0014-5793(96)00659-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Macrophages contain arginase and an inducible NO synthase, demonstrated by using l-arginine, the common substrate, for production of both nitric oxide and urea. Arginase was inhibited by nitrite, the stable end product of NO. This inhibition was non-competitive, and could not be explained by the reaction of nitrite with arginine, or by the irreversible covalent modification of arginase, or by the removal of Mn²⁺, a cofactor of arginase.

【 授权许可】

Unknown