Oxidized low‐density lipoprotein is cytotoxic to human monocyte‐macrophages: protection with lipophilic antioxidants

FEBS Letters

Marchant, Christine E.¹  Law, Nadine S.¹  van der Veen, Carina¹  Carpenter, Keri L.H.¹  Hardwick, Simon J.¹  Mitchinson, Malcolm J.¹ 

[1] Division of Cellular Pathology, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK

关键词: Oxidized LDL;    Monocyte-macrophage (human);    Cytotoxicity;    Atherosclerosis;    Probucol;    α-Tocopherol;   

DOI  :  10.1016/0014-5793(94)01393-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human monocyte-macrophages were incubated for 24 h with low-density lipoprotein (LDL) which had been previously oxidized for varying periods up to 24 h with copper ions, in the presence or absence of dl-α-tocopherol or probucol. The release of radioactivity from cells preloaded with tritiated adenine was used as an assay of toxicity. Toxicity of oxidized LDL increased with duration of copper oxidation and with increasing evidence of lipid oxidation, measured by assay of thiobarbituric acid-reactive substances and by gas chromatography. Oxidation and toxicity were inhibited by dl-α-tocopherol (200 μM) and probucol (50 μM).

【 授权许可】

Unknown