FEBS Letters | |
A delayed and sustained rise of cytosolic calcium is elicited by oxidized LDL in cultured bovine aortic endothelial cells | |
Nègre-Salvayre, Anne1  Fitoussi, Guylène1  Thiers, Jean-Claude1  Salvayre, Robert1  Réaud, Valérie1  Pieraggi, Marie-Thérèse1  | |
[1] Department of Biochemistry, Metabolic Disease Laboratory, Faculty of Medicine in Rangueil, University Paul Sabatier, Toulouse, France | |
关键词: Calcium; Cytotoxicity; Oxidized LDL; Endothelial cell; Atherosclerosis; Quin-2/AM; Fluo-3/AM; | |
DOI : 10.1016/0014-5793(92)80101-L | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Bovine aortic endothelial cells (BAEC) pulsed for 5 h with mildly oxidized low density lipoproteins (LDL), exhibited a broad, sustained and high peak of [Ca2+]i occurring several hours after the end of the pulse and reaching very high [Ca2+]i values (around 2500–3000 nmol/l) and a concomitant drop of cytosolic pH (around 0.2–0.3 pH units) without any loss of cell viability. When BAEC were continuously pulsed with oxidized LDL, the peak of [Ca2+]i was more sustained than in short pulse experiments and was associated with irreversible morphological changes usually associated with cytotoxic events (blebbing) and with a marked loss of viability. The potential involvement of these biochemical and morphological changes in atherogenesis are discussed.
【 授权许可】
Unknown
【 预 览 】
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