A delayed and sustained rise of cytosolic calcium is elicited by oxidized LDL in cultured bovine aortic endothelial cells

FEBS Letters

Nègre-Salvayre, Anne¹  Fitoussi, Guylène¹  Thiers, Jean-Claude¹  Salvayre, Robert¹  Réaud, Valérie¹  Pieraggi, Marie-Thérèse¹ 

[1] Department of Biochemistry, Metabolic Disease Laboratory, Faculty of Medicine in Rangueil, University Paul Sabatier, Toulouse, France

关键词: Calcium;    Cytotoxicity;    Oxidized LDL;    Endothelial cell;    Atherosclerosis;    Quin-2/AM;    Fluo-3/AM;   

DOI  :  10.1016/0014-5793(92)80101-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Bovine aortic endothelial cells (BAEC) pulsed for 5 h with mildly oxidized low density lipoproteins (LDL), exhibited a broad, sustained and high peak of [Ca²⁺]_i occurring several hours after the end of the pulse and reaching very high [Ca²⁺]_i values (around 2500–3000 nmol/l) and a concomitant drop of cytosolic pH (around 0.2–0.3 pH units) without any loss of cell viability. When BAEC were continuously pulsed with oxidized LDL, the peak of [Ca²⁺]_i was more sustained than in short pulse experiments and was associated with irreversible morphological changes usually associated with cytotoxic events (blebbing) and with a marked loss of viability. The potential involvement of these biochemical and morphological changes in atherogenesis are discussed.

【 授权许可】

Unknown