Anti-atherosclerosis potency of Pandanus tectorius fruit rich by trangeretin and ethyl trans-caffeate, and their cytotoxicity against HepG2 cell line

13th Joint Conference on Chemistry

Andriani, Yosie^1 ; Pangestika, Inten^1 ; Oksal, Efriyana^1 ; Mohamad, Habsah^1 ; Amir, Hermansyah^2 ; Muhammad, Tengku Sifzizul Tengku^3 ; Wahid, Mohd Effendi Abd^4

Institute of Marine Biotechnology, Universiti Malaysia Terengganu, Mengabang Telipot, Kuala Nerus, Terengganu

21030, Malaysia^1

Educational Chemistry Program, Faculty of Teacher Training and Education, Bengkulu University, Bengkulu

38371, Indonesia^2

Research Management Center, Universiti Malaysia Terengganu, Mengabang Telipot, Kuala Nerus, Terengganu

21030, Malaysia^3

School of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu, Mengabang Telipot , Kuala Nerus, Terengganu

21030, Malaysia^4

关键词: Adverse side effects;    Atherosclerosis;    Cytotoxic activities;    HepG2;    HMG-CoA reductase;    Hypercholesterolemia;    Pandanus tectorius;    Pharmaceutical market;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/509/1/012155/pdf
DOI  :  10.1088/1757-899X/509/1/012155

来源: IOP

【 摘 要 】

Hypercholesterolemia can be a major contributing agent to the development of atherosclerosis. Atherosclerosis can provoke to heart attack, is a primary reason of death worldwide and current drug statins is not without adverse side effects. This warrants for alternative remedial candidates from natural resources. Natural products from plant play an important role in production of new potential drugs to the pharmaceutical market. Pandanus tectorius (Pandanaceae) is one of the most popular herbs and locally grows in Malaysia and Indonesia is commonly known as 'pandan laut. It has been used traditionally to cure certain diseases. The aimed of study is to find out the potency of fruit rich by tangeretin and ethyl trans-caffeate from P. tectorius for atherosclerosis prevention through inhibiting of the HMG-CoA reductase activity. Since the cholesterol mechanism occurs in the liver, the cytotoxicity activity of all extracts and compounds were necessary analysed. The sample was extracted successively using hexane, ethyl acetate and methanol. The compounds profiling was done by thin layer chromatography (TLC). The HMG-CoA reductase kit for anti-atherosclerosis assay (pravastatin as a controlled drug) was purchased from Sigma Aldrich. The cytotoxicity activity was observed by MTT assay. The both data were read by ELISA reader at wavelength of 340 nm and 570 nm, respectively. Result revealed that all extracts have proven to inhibit the activity of HMG-CoA reductase with the highest activity was obtained by PMK ((9 times higher than pravastatin). All samples also showed no cytotoxic activity against HepG2 cell line since they have IC50 value more than 30 μg/mL. This Study found that P. tectorius fruit rich by tangeretin and ethyl trans-caffeate produces a new candidate for treatment of atherosclerosis.