Purified retinal nitric oxide synthase enhances ADP‐ribosylation of rod outer segment proteins

FEBS Letters

Koch, Karl-Wilhelm¹  Zoche, Martin¹ 

[1] Institut für Biologische Informationsverarbeitung, Forschungszentrum Jülich, Postfach 1913, D-52425 Jülich, Germany

关键词: Retina;    Nitric oxide synthase;    Rod outer segment;    ADP-ribosylation;    retNOS;    retinal nitric oxide synthase;    ROS;    rod outer segments;    OS-IS;    outer segments attached to parts of the inner segments;    PMSF;    phenylmethylsulfonylfluoride;    H4B;    (6R)-5;    6;    7;    8-tetrahydro-l-biopterin;    SNP;    sodium nitroprusside;    SIN-1;    3-morpholinosydnonimine;   

DOI  :  10.1016/0014-5793(94)01355-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nitric oxide synthase is present in different cell layers of vertebrate retina and seems to have neuromodulatory functions in the outer retina. The enzyme, when purified from a bovine retina extract, has an apparent molecular mass of 160 kDa and resembles the neuronal constitutive NOS type I with respect to Ca²⁺-calmodulin sensitivity, K _m value and inhibition by analogues of l-arginine. Retinal NOS is present in a preparation of rod outer segments attached to parts of the inner segments, but not in pure outer segments. We describe the enhancement of specific ADP-ribosylation of outer segment proteins by purified retinal NOS.

【 授权许可】

Unknown