Optical Coherence Tomography and Raman Spectroscopy of the Retina.

Evans, J. W. ; Zawadzki, R. J. ; Liu, R. ; Chan, J. ; Lane, S. ; Werner, J. S.

Technical Information Center Oak Ridge Tennessee

关键词: Retina;    Optical coherence tomography;    Imaging modalities;    Raman spectography;    Biochemical analysis;   

RP-ID  :  DE2009948213

学科分类：工程和技术（综合）

美国|英语

来源: National Technical Reports Library

【 摘 要 】

Imaging the structure and correlating it with the biochemical content of the retina holds promise for fundamental research and for clinical applications. Optical coherence tomography (OCT) is commonly used to image the 3D structure of the retina and while the added functionality of biochemical analysis afforded by Raman scattering could provide critical molecular signatures for clinicians and researchers, there are many technical challenges to combining these imaging modalities. We present an ex vivo OCT microscope combined with Raman spectroscopy capable of collecting morphological and molecular information about a sample simultaneously. The combined instrument will be used to investigate remaining technical challenges to combine these imaging modalities, such as the laser power levels needed to achieve a Raman signal above the noise level without damaging the sample.