Cloning and sequencing of four structural genes for the Na+‐translocating NADH‐ubiquinone oxidoreductase of Vibrio alginolyticus

FEBS Letters

Beattie, P.²  Ward, F.B.²  Bourne, R.M.¹  Leach, D.²  Rich, P.R.¹  Tan, K.² 

[1] Glynn Research Institute, Bodmin, PL30 4AU, UK;Institute for Cell and Molecular Biology, Darwin Building, Edinburgh University, Mayfield Rd, Edinburgh EH9 3JR, UK

关键词: Cloning;    Nucleotide sequence;    NADH—ubiquinone oxidoreductase;    Vibrio alginolyticus;    ORF;    open reading frame;    PAGE;    polyacrylamide gel electrophoresis;    complex I;    H+-translocating NADH-ubiquinone oxidoreductase complex;   

DOI  :  10.1016/0014-5793(94)01275-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Oligonucleotide probes based on the N-terminal amino acid sequences of the NqrA and NqrC subunits were used to clone genes for the Na⁺-dependent NADH-ubiquinone oxidoreductase complex from Vibrio alginolyticus. Four consecutive ORFs were identified encoding subunit proteins of 48.6, 46.8, 27.7 and 22.6 kDa, respectively (NqrA—D). A further ORF, showing 71% homology to the BolA protein of Escherichia coli, was located upstream. From sequence comparisons, we conclude that the Na⁺-dependent NADH-ubiquinone oxidoreductase complex of V. alginolyticus is clearly distinct from the corresponding H⁺-dependent enzymes of both prokaryotes and eukaryotes.

【 授权许可】

Unknown