In Vitro Pathogenicity of Bacterial Brown Band Disease on Acropora sp.

MarSave International Symposium 2018: "Strengthening Marine Resilience for Sustainable Development Goals"

生态环境科学

Rahmi^1 ; Jompa, J.^2 ; Tahir, A.^2

Faculty of Agriculture, Universitas Muhammadiyah, Makassar, Indonesia^1

Faculty of Marine and Fisheries Science, Universitas Hasanuddin, Indonesia^2

关键词: Bacterial cultures;    Bacterial infections;    Bacterial strains;    Histological analysis;    Histological observations;    Microbiology laboratory;    Rate of infections;    Vibrio alginolyticus;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/253/1/012013/pdf
DOI  :  10.1088/1755-1315/253/1/012013

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

This study aimed to determine which bacteria trigger brown band disease (BrB) through assessing the level of pathogenicity of bacteria present and microscopic histological analysis of coral tissue cells. The study was conducted from October 2014 to March 2015. The bacterial culture stage was conducted at the Faculty of Medicine Microbiology Laboratory, Universitas Hasanuddin, the histological analysis was conducted at the, Maros Veterinary Centre Pathology Laboratory, and pathogenicity tests of bacteria from brown band disease on healthy corals were conducted at the Marine Station Hatchery of the Faculty of Marine Science and Fisheries, Universitas Hasanuddin, on Barrang Lompo Island. Pathogenicity tests indicated that post-infection time before the appearance of BrB symptoms was different for each bacteria tested. The fastest-acting bacterial strain was Acinetobacter sp. RA3849 ORF, which caused disease symptoms in 5 hours, followed by Streptococcus sp. YM395 (9 hours), E. faecalis C56 (20 hours), and Vibrio alginolyticus H2X5 (22 hours). Acinetobacter sp. also produced the highest rate of infection spread in Acropora sp., reaching 5.05 cm/day. Histological observation showed tissue damage (necrosis) due to bacterial infection in the epidermal layer and hyperplasia of the gastrodermal layer, where there was an abnormal increase in abnormal tissue or organs due to an increase in the number of cells. The in vitro pathogenicity test showed that Acinetobacter sp. strain RA3849 reacts as a trigger of BBD disease.