Molecular cloning and characterization of the complementary DNA coding for the B‐chain of murine Clq

FEBS Letters

Loos, Michael¹  Reid, Kenneth B.M.²  Petry, Franz¹ 

[1] Institute of Medical Microbiology, Johannes Gutenberg-University, Augustusplatz/Hochhaus, 6500 Mainz, FRG;MRC Immunochemistry Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, England

关键词: Clq;    Cloning;    Nucleotide sequence;    mRNA;   

DOI  :  10.1016/0014-5793(89)81622-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

cDNA clones coding for the B-chain of murine Clq were isolated from a mouse macrophage library. The characterized clones include the total coding region plus a leader sequence. High homology was found with human Clq B-chain in the coding region (81%). Northern blot analysis of total RNA from different tissues of Balb/c mice showed one band of approximately 1.2 kb. The highest signal was found in RNA preparations of thioglycolate-activated peritoneal macrophages. The probe also hybridized with mRNA from spleen, thymus and heart. Extremely weak signals were found in liver, kidney, lung and intestine tissues.

【 授权许可】

Unknown