Isolation of cDNA clones of human argininosuccinate lyase and corrected amino acid sequence

FEBS Letters

Saheki, Takeyori¹  Iwanaga, Sadaaki²  Amaya, Yoshihiro³  Kobayashi, Keiko¹  Tatsuno, Michiko¹  Matuo, Seiji¹  Miyata, Toshiyuki²  Mori, Masataka³ 

[1] Department of Biochemistry, Faculty of Medicine, Kagoshima University, Kagoshima 890, Japan;Department of Biology, Faculty of Science, Kyushu University, Fukuoka 812 Japan;Institute for Medical Genetics, Kumamoto University Medical School, Kumamoto 862, Japan

关键词: Argininosuccinate lyase;    Cloning;    Nucleotide sequence;    Amino acid sequence;    Argininosuccinic aciduria;    ASL;    argininosuccinate lyase;    HPLC;    high-performance liquid chromatography;   

DOI  :  10.1016/0014-5793(88)80124-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the present study, we isolated clones of human argininosuccinate lyase (ASL) cDNA from a liver cDNA library using a clone of rat ASL cDNA and analyzed human ASL cDNA nucleotide sequence. The results reveal that the sequence of human ASL cDNA published by O'Brien et al. in 1986 [Proc. Natl. Acad. Sci USA 83, 7211–7215] had one-base deletions at three independent positions in the coding regions near the COOH-terminus, which caused frame-shift variations in the amino acid sequence. Amino acid sequencing of peptides prepared from purified human liver ASL showed our predicted amino acid sequence to be correct.

【 授权许可】

Unknown