γ‐Subunit of mouse retinal cyclic‐GMP phosphodiesterase: cDNA and corresponding amino acid sequence

FEBS Letters

Farber, Debora B.¹  Tuteja, Narendra¹ 

[1] Department of Ophthalmology and Jules Stein Eye Institute, UCLA School of Medicine, Los Angeles, CA 90024-1771, USA

关键词: Cyclic-GMP phosphodiesterase: cDNA cloning;    Nucleotide sequence;    Amino acid sequence;    (Mouse retina);   

DOI  :  10.1016/0014-5793(88)80413-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cDNA nucleotide and corresponding amino acid sequences of the γ-subunit of cyclic-GMP phosphodiesterase (cGMP-PDE_γ) from mouse retina have been determined. The cDNA translated region was found to be 91.5% homologous to the cDNA coding region for the enzyme from bovine retina [(1986) FEBS Lett. 204, 288–292]. On Northern blots of normal mouse retinal RNAs this cDNA hybridized the cGMP-PDE_γ mRNA which is 900 bp long. The mouse γ-subunit contains 87 amino acid residues which share 97.7% homology with the bovine polypeptide [(1986) FEBS Lett. 204, 288–292]. Only two amino acids have been changed, Ala 8 to Gly and Met 17 to Ile.

【 授权许可】

Unknown