Complete amino acid sequence of human placental 17β‐hydroxysteroid dehydrogenase deduced from cDNA

FEBS Letters

Mäentausta, O.¹  Vihko, R.¹  Peltoketo, H.¹  Isomaa, V.¹ 

[1] Biocenter and Department of Clinical Chemistry, University of Oulu, SF-90220 Oulu, Finland

关键词: Estrogen;    17-Hydroxysteroid dehydrogenase;    Molecular cloning;    Nucleotide sequence;    Amino acid sequence;   

DOI  :  10.1016/0014-5793(88)80548-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

CDNA clones for 17β-hydroxysteroid dehydrogenase (17-HSD; EC 1.1.1.62) were isolated from a placental λgt11 expression library using polyclonal antibodies against placental 17-HSD. The largest cDNA contained 1325 nucleotides, consisting of a short 5′-noncoding segment, a coding segment of 987 nucleotides terminated by a TAA codon, and a 329 nucleotide long 3′-noncoding segment. The open reading frame encoded a polypeptide of 327 amino acid residues with a predicted M _r of 34853. The amino acid sequence of 23 N-terminal amino acids determined from purified 17-HSD agreed with the sequence deduced from cDNA. The deduced amino acid sequence also contained two peptides previously characterized from the proposed catalytic area of placental 17-HSD.

【 授权许可】

Unknown