Molecular cloning of a levocabastine‐sensitive neurotensin binding site

FEBS Letters

Kaghad, Mourad¹  Bonnin, Jacques¹  Vita, Natalio¹  Guillemot, Marie¹  Delpech, Bruno¹  Le Fur, Gerard¹  Ferrara, Pascual¹  Chalon, Pascale¹  Caput, Daniel¹ 

[1] Sanofi Recherche, BP 137, 31676 Labège Cedex, France

关键词: Neurotensin receptor;    Molecular cloning;   

DOI  :  10.1016/0014-5793(96)00397-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A search for sequences homologous to the neurotensin receptor cDNA in a rat hypothalamic library has identified a novel neurotensin receptor (NTR-2). The 1539 bp cDNA encodes a 416 amino acid protein and shows highest homology to the previously cloned neurotensin receptor (NTR-1) (64% homology and 43% identity). Binding and pharmacological studies demonstrate that NTR-2 expressed in COS cells recognizes neurotensin (NT) with high affinity as well as several other agonists and antagonists. However, a fundamental difference was found; unlike NTR-1, NTR-2 recognizes, with high affinity, levocabastine, a histamine H1 receptor antagonist previously shown to compete with NT for low-affinity binding sites in brain.

【 授权许可】

Unknown