Activation of phosphatidylinositol turnover by neurotensin receptors in the human colonic adenocarcinoma cell line HT29

FEBS Letters

Vincent, Jean-Pierre¹  Kitabgi, Patrick¹  Amar, Shimon¹ 

[1] Centre de Biochimie du Centre National de la Recherche Scientifique, Faculté des Sciences, Parc Valrose, 06034 Nice Cedex, France

关键词: Neurotensin receptor;    (Human colonic HT29 cell);    Phosphatidylinositol turnover;    Inositol trisphosphate;    intracellular Ca2+;    TPA;    4β-phorbol 12-myristate 13-acetate;    IP1;    inositol monophosphate;    IP2;    inositol bisphosphate;    IP3;    inositol trisphosphate;   

DOI  :  10.1016/0014-5793(86)80565-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Association of neurotensin to its receptor in HT29 cells increases the intracellular concentration of inositol phosphates. A rapid (20–30 s), transient stimulation of inositol trisphosphate (275% of the basal level) and inositol bisphosphate (420%) is first observed, followed by a slower, stable increase in inositol monophosphate (170%). Half-maximal stimulation of the three inositol phosphates was obtained with 50–100 nM neurotensin. These results indicate that neurotensin is able to regulate intracellular Ca²⁺ levels in HT29 cells by using inositol trisphosphate as a second messenger.

【 授权许可】

Unknown