Phorbol ester induces loss of VIP stimulation of adenylate cyclase and VIP‐binding sites in HT29 cells

FEBS Letters

Vincent, Jean-Pierre¹  Laburthe, Marc¹  Rouyer-Fessard, Christiane¹  Couvineau, Alain¹  Kitabgi, Patrick¹  Bozou, Jean-Claude¹ 

[1] Centre de Biochimie du CNRS, Parc Valrose Université de Nice, Faculté des Sciences, 06034 Nice CédexFrance

关键词: Phorbol ester;    VIP;    Adenylate cyclase;    VIP receptor;    Protein kinase C;    (Human colonic HT29 cell);    PMA;    4β-phorbol 12-myristate 13-acetate;    4α-PDD;    4α-phorbol 12;    13-didecanoate;    VIP;    vasoactive intestinal peptide;    Ns;    stimulatory GTP-binding protein of adenylate cyclase;    IBMX;    isobutylmethylxanthine;   

DOI  :  10.1016/0014-5793(87)81426-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Treatment of HT29 cells with the tumor promoting phorbol ester PMA resulted in an attenuation of VIP-stimulated cAMP production in intact cells and VIP-stimulated adenylate cyclase activity in cell membranes. PMA did not decrease the ability of cholera toxin and forskolin to elevate cAMP levels in intact cells. Fluoride-stimulated adenylate cyclase activity in HT29 cells homogenates was not affected byPMA. The maximal VIP binding capacity of homogenates prepared from HT29 cells treated with PMA was decreased by 50%. It is concluded that protein kinase C regulates VIP receptor function possibly through phosphorylation of the VIP receptor.

【 授权许可】

Unknown