Sequencing and the alignment of structural genes in the nqr operon encoding the Na+‐translocating NADH‐quinone reductase from Vibrio alginolyticus

FEBS Letters

Hayashi, Maki¹  Unemoto, Tsutomu¹  Hirai, Kouji¹ 

[1] Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263, Japan

关键词: Na+ pump;    NADH-quinone reductase;    Nucleotide sequence;    nqr operon;    Vibrio alginolyticus;   

DOI  :  10.1016/0014-5793(95)00283-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We previously cloned a part of nqr operon encoding the Na⁺-translocating NADH-quinone reductase (NQR) from the marine Vibrio alginolyticus (Hayashi et al., FEBS Lett. 356 (1994) 330–332]. From its nucleotide sequences, four consecutive open reading frames (ORF) encoding the γ-subunit (27.7 kDa), two unidentified ORFs of 22.6 kDa and 21.5 kDa, and the β-subunit (45.3 kDa) were recognized. The gene encoding the α-subunit was located upstream, and together with the recent report by Beattie et al. [FEBS Lett. 356 (1994) 333–338], the nqr operon was found to be constructed from six consecutive structural genes, where nqrl, nqr3 and nqr6 correspond to the α-, γ-, and β-subunits, respectively, of the NQR complex.

【 授权许可】

Unknown