Basepairing with 18S ribosomal RNA in internal initiation of translation

FEBS Letters

Voorma, Harry O.¹  Thomas, Adri A.M.¹  Scheper, Gert C.¹ 

[1] Department of Molecular Cell Biology, Padualaan 8, 3584 CH Utrecht, The Netherlands

关键词: Picornavirus;    Translation;    Internal initiation;    Polypyrimidine tract;    18S ribosomal RNA;    Basepairing;    AUGy;    AUG codon located approximately 20 nucleotides downstream of conserved polypyrimidine tract;    eIF;    eukaryotic initiation factor;    EMC;    encephalomyocarditisvirus;    FMDV;    foot-and-mouth disease virus;    IRES;    internal ribosomal entry site;    HAV;    hepatitis A virus;    HRV;    human rhinovirus;    poly Y;    polypyrimidine tract;    PTB;    polypyrimidine tract binding protein;    5′UTR;    5′ untranslated region;   

DOI  :  10.1016/0014-5793(94)00975-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In concert with the translation initiation factors ‘trans-acting’ factors function specifically during internal initiation on picornaviral mRNAs. Of these trans-acting factors, two have been identified as the La-protein and the polypyrimidine tract binding protein. Within the internal ribosomal entry site on the viral RNA, sequences are present that direct the ribosome to the initiation codon. We suggest that selection of the correct AUG initiation codon occurs through basepairing with a part of 18S ribosomal RNA.

【 授权许可】

Unknown