A factor that specifically binds to the 5'‐untranslated region of encephalomyocarditis virus RNA

FEBS Letters

Borovjagin, A.V.¹  Ugarova, T.Yu.²  Evstafieva, A.G.¹  Shatsky, I.N.¹ 

[1] Divisions of Chemistry and Biochemistry of Nucleoproteins, A.N. Belozersky Laboratory, Moscow State University, 119899 Moscow, USSR;Virus-Cell Interactions, A.N. Belozersky Laboratory, Moscow State University, 119899 Moscow, USSR

关键词: Translation;    Internal initiation;    Encephalomyocarditis virus;    Protein-RNA interaction;    EMCV;    encephalomyocarditis virus;    AIMV;    alfalfa mosaic virus;    5'-UTR;    5'-untranslated region;    tr;    transcript;    nt;    nucleotide;    PAAG;    polyacrylamide gel;   

DOI  :  10.1016/0014-5793(90)80561-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A protein factor that specifically binds to the 5'-untranslated region of encephalomyocarditis virus (EMCV) RNA has been found in extracts of ascites carcinoma Krebs-2 cells. This was done using UV-irradiation on extracts supplemented with in vitro synthesized ³²P-labelled transcripts followed by analysis of crosslinked proteins by SDS-polyacrylamide gel electrophoresis. The transcripts represented the viral RNA sequence from nt 315 to 1155, its derivatives with internal deletions or truncated forms. This set of transcripts has allowed us to find out that the factor (p58) binds to EMCV RNA within the sequence 315–485.

【 授权许可】

Unknown