Porcine polypyrimidine tract‐binding protein stimulates translation initiation at the internal ribosome entry site of foot‐and‐mouth‐disease virus

FEBS Letters

Niepmann, Michael¹ 

[1] Institut für Biochemie, Friedrichstrasse 24, D-35392 Giessen, Germany

关键词: Translation;    Picornavirus;    Foot-and-mouth-disease virus;    Polypyrimidine tract-binding protein;   

DOI  :  10.1016/0014-5793(96)00509-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cDNA for porcine polypyrimidine tract-binding protein (sPTB) was cloned. The sPTB amino acid sequence is highly homologous to the human PTB sequence (97% identity), and the sPTB sequence corresponds to that of the longest human PTB, PTB4. The specificity of binding in the UV-crosslink of sPTB to the internal ribosome entry site (IRES) of foot-and-mouth-disease virus (FMDV) is similar to that of human PTB. Purified recombinant sPTB efficiently stimulates internal translation initiation directed by the FMDV IRES in a rabbit reticulocyte lysate translation system from which the internal PTB had been depleted.

【 授权许可】

Unknown