Polypyrimidine tract‐binding protein interacts with HnRNP L

FEBS Letters

Jang, Sung K¹  Kim, Jung-E¹  Cho, Ook H¹  Hahm, Bumsuk¹  Kim, Jong H¹  Oh, Young L¹  Kim, Yoon K¹ 

[1] Department of Life Science, Pohang University of Science and Technology, San31, Hyoja-Dong, Pohang, Kyungbuk 790-784, South Korea

关键词: Polypyrimidine tract-binding protein;    Heterogeneous nuclear ribonucleoprotein L;    Protein-protein interaction;   

DOI  :  10.1016/S0014-5793(98)00269-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Polypyrimidine tract-binding protein (PTB) is involved in pre-mRNA splicing and internal ribosomal entry site (IRES)-dependent translation. In order to identify cellular protein(s) interacting with PTB, we performed a yeast two-hybrid screening. Heterogeneous nuclear ribonucleoprotein L (hnRNP L) was identified as a PTB-binding protein. The interaction between PTB and hnRNP L was confirmed in an in vitro binding assay. Both PTB and hnRNP L were found to localize in the nucleoplasm, excepting the nucleoli, in HeLa cells by the green fluorescent protein (GFP)-fused protein detection method. The N-terminal half of PTB (aa 1–329) and most of hnRNP L (aa 141–558) is required for the interaction between PTB and hnRNP L.

【 授权许可】

Unknown