期刊论文详细信息
FEBS Letters | |
Internal ribosome entry site ofencephalomyocarditis virus RNA is unable to direct translation in Saccharomyces cerevisiae | |
Borovjagin, A.V.1  Beletsky, A.V.1  Evstafieva, A.G.1  Bogdanov, A.A.1  | |
[1] A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, 119899 Moscow, Russian Federation | |
关键词: Translation; Internal initiation; Encephalomyocarditis virus; Prothymosin α; Saccharomyces cerevisiae; E. coli; Escherichia coli; EMCV; encephalomyocarditis virus; 1RES; internal ribosome entry site; S.cerevisiae; Saccharomyces cerevisiae; PAAG; polyacrylamide gel; nt(s); nucleotide(s); PAAG; polyacrylamide gel(s); PCR; polymerase chain reaction; PTB; ‘pyrimidine tract binding protein’; | |
DOI : 10.1016/0014-5793(93)80745-G | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
To evaluate the potential of the encephalomyocarditis virus (EMCV) internal ribosome entry site (1RES) to promote efficient expression of foreign genes in the yeast, S. cerevisiae, we have constructed E.coli-yeast shuttle vectors in which the EMCV 5' non-coding region was fused to the reporter gene, human prothymosin α. Efficiency of translation of corresponding RNA transcripts in mammalian cell-free systems was highly dependent on the sequence context and/or position of the initiation codon. No translation of these IRES-dependent mRNAs occurred in S. cerevisiae.
【 授权许可】
Unknown
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