Internal ribosome entry site ofencephalomyocarditis virus RNA is unable to direct translation in Saccharomyces cerevisiae

FEBS Letters

Borovjagin, A.V.¹  Beletsky, A.V.¹  Evstafieva, A.G.¹  Bogdanov, A.A.¹ 

[1] A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, 119899 Moscow, Russian Federation

关键词: Translation;    Internal initiation;    Encephalomyocarditis virus;    Prothymosin α;    Saccharomyces cerevisiae;    E. coli;    Escherichia coli;    EMCV;    encephalomyocarditis virus;    1RES;    internal ribosome entry site;    S.cerevisiae;    Saccharomyces cerevisiae;    PAAG;    polyacrylamide gel;    nt(s);    nucleotide(s);    PAAG;    polyacrylamide gel(s);    PCR;    polymerase chain reaction;    PTB;    ‘pyrimidine tract binding protein’;   

DOI  :  10.1016/0014-5793(93)80745-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To evaluate the potential of the encephalomyocarditis virus (EMCV) internal ribosome entry site (1RES) to promote efficient expression of foreign genes in the yeast, S. cerevisiae, we have constructed E.coli-yeast shuttle vectors in which the EMCV 5' non-coding region was fused to the reporter gene, human prothymosin α. Efficiency of translation of corresponding RNA transcripts in mammalian cell-free systems was highly dependent on the sequence context and/or position of the initiation codon. No translation of these IRES-dependent mRNAs occurred in S. cerevisiae.

【 授权许可】

Unknown