Heat‐shock response in a yeast tps1 mutant deficient in trehalose synthesis

FEBS Letters

Argüelles, Juan Carlos¹ 

[1] Departamento de Genética y Microbiología, Facultad de Biología, Universidad de Murcia, E-30071 Murcia, Spain

关键词: Heat shock;    Trehalose;    Thermotolerance;    Saccharomyces cerevisiae;    Trehalase;    Trehalose-6P synthase;   

DOI  :  10.1016/0014-5793(94)00786-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Exponential cells of the Saccharomyces cerevisiae tps1 mutant underwent a rapid loss of viability upon a non-lethal heat exposure (from 28 to 42°C). However, a further more severe heat stress (52.5°C 5 min) induced an increase in the fraction of viable cells. This mutant can not synthesize trehalose either at 28° C or at 42°C due to the lack of a functional trehalose-6P synthase complex. In control experiments carried out with the wild-type W303-1 B, heat-stressed exponential phase cultures grown on YPgal at 28°C acquired thermotolerance to a higher extent than identical cultures grown on YPD, although in both cultures the level of stored trehalose was negligible. These data suggest that the bulk of trehalose accumulated in yeast upon mild heat treaments is not sufficient to account for the acquisition of thermotolerance.

【 授权许可】

Unknown