Heat shock induces enzymes of trehalose metabolism, trehalose accumulation, and thermotolerance in Schizosaccharomyces pombe, even in the presence of cycloheximide

FEBS Letters

Hottiger, Thomas¹  De Virgilio, Claudio¹  Simmen, Urs¹  Boiler, Thomas¹  Wiemken, Andres¹ 

[1] Department of Botany, University of Basel, Basel, Switzerland

关键词: Trehalose;    Heat shock;    Thermotolerance;    Trehalase;    Trehalose-6-phosphate synthase;    Schizosaccharomyces pombe;   

DOI  :  10.1016/0014-5793(90)81062-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Exponentially growing cells of the fission yeast, Schizosaccharomyces pombe, contained virtually no trehalose at 27°C but rapidly accumulated large quantities during heat shock at 40°C. Activities of trehalose-6-phosphate synthase and trehalase also increased upon heat shock. Thermotolerance of the cells, measured as survival at 52°C, increased in parallel to trehalose accumulation and decreased in parallel to the trehalose levels when cells were shifted back to 27°C. Trehalose levels, activities of enzymes of trehalose metabolism and thermotolerance strongly increased upon heat shock even in the presence of cycloheximide, indicating that none of these effects requires protein synthesis. The data support the hypothesis that trehalose acts as a thermoprotectant in Schizosaccharomyces pombe.

【 授权许可】

Unknown