Development of Lyoprocessing Technique to Enhance Biological Storage Outcomes

Dry preservation;Anhydrobiosis;Desiccation;Surface tension;Lyopreservation;Bioenergetics;Trehalose;Raman spectroscopy;Cellular membrane;Mechanical engineering;Biomedical engineering;Mechanical Engineering, College of Engineering & Computer Science

Solocinski, JasonMiller, John ;

University of Michigan

关键词: Dry preservation;    Anhydrobiosis;    Desiccation;    Surface tension;    Lyopreservation;    Bioenergetics;    Trehalose;    Raman spectroscopy;    Cellular membrane;    Mechanical engineering;    Biomedical engineering;    Mechanical Engineering, College of Engineering & Computer Science;   

Others  :  https://deepblue.lib.umich.edu/bitstream/handle/2027.42/143527/MasterThesis_Solocinski2.pdf?sequence=1&isAllowed=y

瑞士|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

Dry state preservation at ambient temperatures (lyopreservation) is a biomimetic alternative to low temperature stabilization (cryopreservation) of biological materials.Lyopreservation is hypothesized to rely upon the creation of a glassy environment, which is commonly observed in desiccation-tolerant organisms. Non-uniformities in driedsamples have been indicated as one of the reasons for instability in storage outcomes.The current study presents a simple, fast, and uniform surface tension based technique that can be implemented for lyopreservation of mammalian cells. The technique involves withdrawing cells attached to rigid substrates to be submerged in a solution of lyoprotectant and then withdrawing the samples at a specific rate to an inert environment. This creates a uniform thin film of desiccated lyoprotectant. The residual moisture contents at different locations in the desiccated film is quantified using a spatially resolvedRaman microspectroscopy technique. Post-desiccation cellular viability and growth is quantified using fluorescent microscopy and dye exclusion assays. Cellular injury following desiccation is evaluated by bioenergetic quantification of metabolic functionsusing extracellular flux analysis and by a Raman microspectroscopic analysis of change in membrane structure. The technique developed here addresses an important bottleneckof lyoprocessing which requires the fast and uniform desiccation of cellular samples.