Effects of heat shock on the level of trehalose and glycogen, and on the induction of thermotolerance in Neurospora crassa

FEBS Letters

François, Jean M.¹  Jorge, Joâo A.²  Terenzi, Héctor F.²  Neves, Maria José² 

[1] Laboratoire de Chimie Physiologique, International Institute of Cellular and Molecular Pathology and Catholic University of Louvain, Brussels, Belgium;Departmento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo - 14049 Ribeirão Preto, São Paulo, Brazil

关键词: Heat shock;    Thermotolerance;    Trehalose;    Glycogen;    Cyclic AMP: Neurospora crassa;   

DOI  :  10.1016/0014-5793(91)80544-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Neurospora crassa conidiospore germlings exposed to a heat shock (30–45°C) rapidly accumulated trehalose and degraded glycogen, even in the presence of cycloheximide. This phenomenon was also rapidly reversible upon return of the cells at 30°C. Trehalose accumulation at 45°C demanded an exogenous source of carbon and either glucose or glycerol fulfilled such requirement. Experiments with the cyclic AMP-deficient cr-1 mutant suggested that the effects of temperature shifts on trehalose level were independent of cAMP metabolism. Cells exposed at 45°C under conditions permissive for trehalose accumulation (i.e. in the presence of an assimilable carbon source) also acquired thermotolerance.

【 授权许可】

Unknown