Enzymatic synthesis of a novel trehalose derivative, 3,3′‐diketotrehalose, and its potential application as the trehalase enzyme inhibitor

FEBS Letters

Akaike, Eri¹  Sode, Koji¹  Tsugawa, Wakako¹  Sugiura, Hiroto¹ 

[1] Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Nakamachi, Koganei, Tokyo 184-8588, Japan

关键词: Glucose-3-dehydrogenase;    Trehalose;    Trehalase;    Inhibitor;    Insecticide;   

DOI  :  10.1016/S0014-5793(00)02427-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We reported the preparation of a novel trehalose derivative based on enzymatic oxidation of trehalose by water-soluble glucose-3-dehydrogenase (G3DH) from marine bacterium Halomonas sp. α-15 cells. The product of G3DH enzymatic conversion was 3,3′-diketotrehalose (3,3′dkT), a novel trehalose derivative of which both third hydroxy groups of glucopyranosides were oxidized. 3,3′dkT was revealed to show an inhibitory effect toward pig-kidney and Bombyx mori trehalases. The IC₅₀ values of 3,3′dkT were 0.8 and 2.5 mM and K _i values were 0.2 and 0.6 mM for pig-kidney and for B. mori trehalases, respectively. In addition, 3,3′dkT did not show any inhibitory effect on both maltase and mannosidase activities. Therefore, 3,3′dkT was a specific inhibitor of trehalases.

【 授权许可】

Unknown