Identification and characterisation of a type‐1 protein phosphatase from the okadaic acid‐producing marine dinoflagellate Prorocentrum lima

FEBS Letters

Holmes, Charles F.B.¹  Boland, Marion P.¹  Taylor, Max F.J.R.² 

[1] MRC Protein Structure/Function Group, Department of Biochemistry, University of Alberta, Edmonton, T6G 2H7, Canada;Department of Oceanography, University of British Columbia, Vancouver, V6T 1Z4, Canada

关键词: Protein phosphatase-1;    Marine dinoflagellate;    Prorocentrum lima;    Okadaic acid;    Tumour promoter;    Protein phosphatase inhibitor;   

DOI  :  10.1016/0014-5793(93)81670-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The unicellular marine dinoflagellate, Prorocentrum lima, an established producer of okadaic acid (OA), was shown to contain a type-1 protein phosphatase (PP-1) the biochemical profile of which on Mono-Q and Superdex-75 fast protein liquid chromatography was identical to the catalytic subunit of PP-1 from rabbit skeletal muscle. Purified P. lima PP-1 (apparent molecular mass 37.5 kDa) was highly sensitive to inhibition by mammalian protein phosphatase inhibitor-1 and inhibitor-2, and to OA itself. A 6–7-fold increase in OA production by P. lima, when grown under controlled conditions, correlated with an up to 300-fold increase in P. lima PP-1 activity. Furthermore, P. lima did not contain any detectable type-2A protein phosphatase activity. This study represents the first identification of a serine/threonine protein phosphatase in a dinoflagellate.

【 授权许可】

Unknown