DARPP‐32 (dopamine and cAMP‐regulated phosphoprotein, M r 32,000) is a membrane protein in the bovine parathyroid

FEBS Letters

Kinder, Barbara K.¹  Hemmings, Hugh C.²  Matovcik, Lisa M.¹ 

[1] Department of Surgery, West Haven Department of Veterans Affairs Medical Center, and Yale University School of Medicine, New Haven, CT 06510-8062, USA;Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, 1230 York Ave., New York, NY 10021, USA

关键词: Parathyroid;    DARPP-32;    PTH secretion;    Protein phosphatase-1;   

DOI  :  10.1016/0014-5793(95)00359-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A distinct form of DARPP-32, a protein phosphatase-1 inhibitor, has been identified in bovine calf parathyroid glands. Immunoblot analysis of parathyroid tissue revealed a 32 kDa protein present predominantly in a particulate fraction; it remained particulate after treatment with 1.0 M NaCl or 0.1 M Na₂CO₃. Metabolic labeling of parathyroid cells with mevalonolactone demonstrated that DARPP-32 is isoprenylated. Immunocytochemical localization studies demonstrated that DARPP-32 is present in vesicles throughout the cytoplasm of parathyroid cells, and that protein phosphatase-1γ is concentrated in the region of the plasma membrane. Thus, in contrast to the predominately soluble form of DARPP-32 that has been characterized in selected areas of the central nervous system, the parathyroid form is tightly associated with intracellular membranes.

【 授权许可】

Unknown