Parathyroid hormone secretion in the absence of extracellular free Ca2+ and transmembrane Ca2+ influx

FEBS Letters

Scarpa, Antonio¹  Pintado, Elizabeth¹  Wallace, Jean¹ 

[1] Department of Biochemistry and Biophysics, University of Pennsylvania, School of Medicine, Philadelphia, PA 19104, USA

关键词: Calcium channels;    Calcium influx;    Cell isolation;    Exocytosis;    Parathyroid cell;    PTH secretion;    PTH;    parathyroid hormone;    Hepes;    N-2-hydroxyethylpiperazine-N-ethane sulfonic acid;    RIA;    radioimmunoassay;    NaCN;    sodium cyanide;    BSA;    bovine serum albumin;    EGTA;    ethylene glycol-bis(β-aminoethyl ether)N;    N-tetraacetic acid;   

DOI  :  10.1016/0014-5793(83)80348-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The involvement of extracellular Ca²⁺ and Ca²⁺ influx across the plasma membrane in parathyroid hormone (PTH) secretion was investigated in vitro using a new preparation of bovine parathyroid cells. Incubation of these cells in the presence of 25 μM or 2.5 μM free ambient Ca²⁺ induced a maximal rate of PTH secretion. Low free Ca²⁺ secretion is not associated with changes in membrane permeability, requires metabolic energy, and is reversible. The Ca²⁺ channel blocker D600 had no effect on either ⁴⁵Ca-influx or PTH secretion in these cells. These results, showing that extracellular Ca²⁺ and Ca²⁺ influx across the plasma membrane are not required for PTH secretion by parathyroid cells, emphasize the differences in the cellular mechanisms underlying the secretion of PTH vs that of other secretory cells.

【 授权许可】

Unknown