Early transmembrane events in tumour cell responses observed by stopped‐flow fluorometry

FEBS Letters

Nakanishi, Mamoru¹  Utsunomiya, Naoko¹  Tsuboi, Masamichi¹ 

[1] Faculty of Pharmaceutical Sciences, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113, Japan

关键词: Stopped-flow fluorometry;    Membrane fluidity;    Calcium influx;    MHC antigen;    Con A;    Tumor cell;    MHC;    major histocompatibility complex;    Con A;    concanavalin A;    CTC;    chlortetracycline;    PBS;    phosphate-buffered saline;    FCS;    fetal calf serum;   

DOI  :  10.1016/0014-5793(85)81251-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Early transmembrane events of tumour cells (mouse myeloma X5563 and lymphoma RDM4) after binding of a monoclonal antibody against mouse MHC antigen and a mitogenic lectin, Con A, were examined by stopped-flow fluorometry with 3 different fluorescent probes. The results showed that membrane fluidities of the cells increased first after binding of anti H-2K^k monoclonal antibody (11-4.1), then calcium was released from intracellular stores into the cytoplasma, and lastly calcium influx occurred from the external medium into the cytoplasma. While Con A only induced calcium influx from the external medium into the cytoplasma.

【 授权许可】

Unknown