Modulation of functional activities of the neurotoxin from black widow spider venom

FEBS Letters

Storchak, L.G.²  Pozdnyakova, N.G.²  Grishin, E.V.¹  Himmelreich, N.H.²  Volkova, T.M.¹  Pluzhnikov, K.A.¹  Woll, P.G.¹ 

[1] Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16110, 117871 GSP Moscow, V-437, Russian Federation;Palladin Institute of Biochemistry, Ukranian Academy of Sciences, ul. Leontovich 9, Kiev 252030, Ukraine

关键词: α-Latrotoxin;    Rat brain synaptosome;    PC12 cell;    Calcium influx;    GABA release;   

DOI  :  10.1016/0014-5793(93)80803-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have studied the action of an α-latrotoxin (α-LTX) complex of two polypeptides (LTX 130 kDa and low molecular weight protein (LMWP) 8 kDa) and the action of a venom fraction containing LTX with excess LMWP on calcium influx into synaptosomes and PC 12 cells as well as on [¹⁴C[GABA release from synaptosomes. Both preparations considerably activate calcium influx and stimulate [¹⁴C]GABA release from synaptosomes. Preincubation of both preparations with antibodies against a 14 amino acid residue C-terminal peptide of LMWP differentially modulates these effects. Antibodies inhibit induced calcium influx and enhance induced GABA release.

【 授权许可】

Unknown