δ‐Atracotoxins from Australian funnel‐web spiders compete with scorpion α‐toxin binding on both rat brain and insect sodium channels

FEBS Letters

Gordon, Dalia²  Little, Michelle J¹  Nicholson, Graham M¹  Cestèle, Sandrine³  Wilson, Harry¹  Martin-Eauclaire, Marie-France³  Tyler, Margaret I⁴  Zappia, Cathy¹ 

[1] Department of Health Sciences, University of Technology, Sydney, Broadway, N.S.W. 2007, Australia;CEA, C.E.-Saclay, Département d'Ingénierie et d'Études des Protéines, Gif-sur-Yvette, F-91911, France;UNRS 6560 CNRS, Laboratoire de Biochimie, Université de la Mediterranée, I.F.R. Jean Roche, 13916 Marseille Cedex 20, France;Deakin Research Ltd., CSIRO Division of Food Processing, North Ryde, N.S.W. 2113, Australia

关键词: Funnel-web spider toxin;    δ-Atracotoxin;    Sodium channel;    Scorpion toxin;    Rat brain synaptosome;    Cockroach neuronal membrane;    δ-Atracotoxin-Ar1 (formerly robustoxin) from Atrax robustus;    δ-Atracotoxin-Hv1 (formerly versutoxin) from Hadronyche versuta;    AaH II;    α-toxin from the venom of the scorpion Androctonus australis hector;    LqhαIT;    α-toxin highly active on insects from the venom of the scorpion Leiurus quinquestriatus hebraeus;    TFA;    trifluoroacetic acid;    RP-HPLC;    reverse phase high performance liquid chromatography;    SDS-PAGE;    sodium dodecyl sulfate polyacrylamide gel electrophoresis;    HEPES;    N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid;    Tris;    2-amino-2-(hydroxymethyl) propane-1;    3-diol;    [3H]STX;    [3H]saxitoxin;    [3H]BTX;    [3H]batrachotoxinin A-20α-benzoate;    BSA;    bovine serum albumin;    AaIT;    excitatory insect-selective toxin from the venom of the scorpion Androctonus australis hector;   

DOI  :  10.1016/S0014-5793(98)01378-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

δ-Atracotoxins are novel peptide toxins from the venom of Australian funnel-web spiders that slow sodium current inactivation in a similar manner to scorpion α-toxins. To analyse their interaction with known sodium channel neurotoxin receptor sites we determined their effect on scorpion toxin, batrachotoxin and saxitoxin binding. Nanomolar concentrations of δ-atracotoxin-Hv1 and δ-atracotoxin-Ar1 completely inhibited the binding of the scorpion α-toxin AaH II to rat brain synaptosomes as well as the binding of LqhαIT, a scorpion α-toxin highly active on insects, to cockroach neuronal membranes. Moreover, δ-atracotoxin-Hv1 cooperatively enhanced batrachotoxin binding to rat brain synaptosomes in an analogous fashion to scorpion α-toxins. Thus the δ-atracotoxins represent a new class of toxins which bind to both mammalian and insect sodium channels at sites similar to, or partially overlapping with, the receptor binding sites of scorpion α-toxins.

【 授权许可】

Unknown