Toxicity and Growth Assessments of Three Thermophilic Benthic Dinoflagellates (Ostreopsis cf. ovata, Prorocentrum lima and Coolia monotis) Developing in the Southern Mediterranean Basin

Toxins

Mohamed Laabir¹  Estelle Masseret¹  Eric Abadie¹  Habiba Nouri²  Nicolas Chomérat³  Manoella Sibat⁴  Zouher Amzil⁴  Ons Kéfi-Daly Yahia⁵  Hela Ben-Gharbia⁵  Habiba Zmerli Triki⁵ 

[1] Center for Marine Biodiversity, Exploitation and Conservation (MARBEC), Montpellier University, Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD), Institut Français de Recherche pour l’Exploitation de la Mer (IFREMER), Place Eugène Bataillon, CC093, Montpellier Cedex 5 F-34095, France;Institut de Recherche pour le Développement (IRD), 2 Rue Des Sports-El Menzah 1, BP 434, Tunis 1004, Tunisia;Laboratoire Environnement Ressource de Bretagne Occidentale (LER-BO), Institut Français de Recherche pour l’Exploitation de la Mer (IFREMER), Station de Biologie Marine, Place de la Croix, BP 40537, Concarneau F-29185, France;Phycotoxins Laboratory, Institut Français de Recherche pour l’Exploitation de la Mer (IFREMER), Rue De l’Ile d’Yeu, BP 21105, Nantes Cedex 3 F-44311, France;Research Group on Oceanography and Plankton Ecology, Tunisian National Institute of Agronomy (INAT), IRESA—Carthage University, 43 Avenue Charles Nicolle, Tunis 1082, Tunisia;

关键词: Ostreopsis cf.ovata;    Prorocentrum lima;    Coolia monotis;    Southern Mediterranean Sea;    growth;    toxicity;   

DOI  :  10.3390/toxins8100297

来源: DOAJ

【 摘 要 】

Harmful benthic dinoflagellates, usually developing in tropical areas, are expanding to temperate ecosystems facing water warming. Reports on harmful benthic species are particularly scarce in the Southern Mediterranean Sea. For the first time, three thermophilic benthic dinoflagellates (Ostreopsis cf. ovata, Prorocentrum lima and Coolia monotis) were isolated from Bizerte Bay (Tunisia, Mediterranean) and monoclonal cultures established. The ribotyping confirmed the morphological identification of the three species. Maximum growth rates were 0.59 ± 0.08 d−1 for O. cf. ovata, 0.35 ± 0.01 d−1 for C. monotis and 0.33 ± 0.04 d−1 for P. lima. Toxin analyses revealed the presence of ovatoxin-a and ovatoxin-b in O. cf. ovata cells. Okadaic acid and dinophysistoxin-1 were detected in P. lima cultures. For C. monotis, a chromatographic peak at 5.6 min with a mass m/z = 1061.768 was observed, but did not correspond to a mono-sulfated analogue of the yessotoxin. A comparison of the toxicity and growth characteristics of these dinoflagellates, distributed worldwide, is proposed.

【 授权许可】

Unknown