Early steps of the P‐glycoprotein expression in cell cultures studied with vital fluorochrome

FEBS Letters

Stromskaya, T.P.¹  Frolova, E.A.¹  Egudina, S.V.¹  Stavrovskaya, A.A.¹ 

[1] Institute of Carcinogenesis, Cancer Research Center of Russian Academy of Medical Sciences, Moscow, Russian Federation

关键词: Multidrug resistance;    P-glycoprotein;    Rhodamin 123;    CH;    colchicine;    CML;    chronic myeloid leukemia;    Mab;    monoclonal antibodies;    MDR;    multidrug resistance;    PBL;    peripheral blood lymphocytes;    PE;    Phycoerythrin;    Pgp;    P-glycoprotein;    Rh123;    Rhodamin 123;   

DOI  :  10.1016/0014-5793(93)80194-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

This study shows that flow cytometry analysis of the rate of fluorochrome Rh123 efflux may be used for detection of cells at initial steps of P-glycoprotein expression and of minor subpopulations of multidrug-resistant (MDR) variants in human cell lines. This method also evaluates the fraction of low-level MDR cells among peripheral blood leukocytes of patients with chronic myeloid leukemia. The alterations in Pgp function were revealed in rat hepatoma cells after short treatment with colchicine.

【 授权许可】

Unknown