期刊论文详细信息
FEBS Letters | |
Early steps of the P‐glycoprotein expression in cell cultures studied with vital fluorochrome | |
Stromskaya, T.P.1  Frolova, E.A.1  Egudina, S.V.1  Stavrovskaya, A.A.1  | |
[1] Institute of Carcinogenesis, Cancer Research Center of Russian Academy of Medical Sciences, Moscow, Russian Federation | |
关键词: Multidrug resistance; P-glycoprotein; Rhodamin 123; CH; colchicine; CML; chronic myeloid leukemia; Mab; monoclonal antibodies; MDR; multidrug resistance; PBL; peripheral blood lymphocytes; PE; Phycoerythrin; Pgp; P-glycoprotein; Rh123; Rhodamin 123; | |
DOI : 10.1016/0014-5793(93)80194-Y | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
This study shows that flow cytometry analysis of the rate of fluorochrome Rh123 efflux may be used for detection of cells at initial steps of P-glycoprotein expression and of minor subpopulations of multidrug-resistant (MDR) variants in human cell lines. This method also evaluates the fraction of low-level MDR cells among peripheral blood leukocytes of patients with chronic myeloid leukemia. The alterations in Pgp function were revealed in rat hepatoma cells after short treatment with colchicine.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020298311ZK.pdf | 345KB | download |