Relation of polymorphism C1236T and C3435T in ABCB1 gene with bone marrow suppression in chemotherapy-treated breast cancer patients

1st International Conference on Tropical Medicine and Infectious Diseases (ICTROMI) Faculty of Medicine Universitas Sumatera Utara

Syarifah, S.^1 ; Hamdi, T.^2 ; Widyawati, T.^1 ; Sari, M.I.^3 ; Anggraini, D.R.^4

Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia^1

Department of Anesthesiology and Incentive Therapy, Faculty of Medicine, Universitas Sumatra Utara, Medan, Indonesia^2

Department of Biochemistry, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia^3

Department of Anatomy, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia^4

关键词: Analysis process;    Breast Cancer;    Efflux pumps;    Genotype frequencies;    Hardy-Weinberg equilibrium;    Indonesians;    P-glycoprotein;    Transmembranes;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/125/1/012126/pdf
DOI  :  10.1088/1755-1315/125/1/012126

来源: IOP

【 摘 要 】

ABCB1 is agene that encoded P-glycoprotein (P-gp), a transmembrane active efflux pump for a variety of carcinogens and cytostatics.ABCB1 polymorphisms C1236T and C3435T contribute to the variability oftherapeutic outcome and side effects.The present study was conducted to investigatethe relation of C1236T and C3435T polymorphisms in ABCB1 gene with bone marrow suppression in breast cancer patients treated withchemotherapy72 Indonesian womens isolated DNA sampleswere amplified using the PCR method. The analysis process of ABCB1 C1236T and C3435T polymorphism was by using thePCR-RFLP method. The frequencies of ABCB1 C1236T genotype for homozygous CC,heterozygous CT and variant TT was 11(15.28%), 42(58.33%), 19(26.39%), respectively. No associationwas between ABCB1 C1236T and C3435T polymorphisms in both individually and haplotypes with bone marrow suppression event (p > 0.05). There was no specific deviation of allele and genotype frequency from Hardy-Weinberg Equilibrium. There was a linkage between heterozygous CT-heterozygous CT in position 1236 and 3435 within 25 people (35%).